The Relationships between Substrates and Enzymes of Glycolysis in Brain

Abstract

In the preceding paper (1) are recorded the concentrations in mouse brain of the substrates and cofactors of the Embden Meyerhof pathway, and the changes in concentration when the glycolytic rate is increased many fold by sudden ischemia (decapitation). In this paper, the concentrations found are examined in relationship to the activity and kinetic properties of each enzyme concerned and the glycolytic flux. To make this possible, the activities of all enzymes of glycolysis in mouse brain except triosephosphate isomerase were measured under conditions designed to simulate the pH and ionic environment of the cell, and abbreviated kinetic studies were made of each enzyme under these same conditions.