The Measurement of Pyridine Nucleotides by Enzymatic Cycling

Abstract

In 1935 Negelein and Haas described a method for the determination of glucose 6-phosphate dehydrogenase activity based on measurement of the rate of reduced triphosphopyridine nucleotide formation from triphosphopyridine nucleotide (1). Since that time innumerable enzymes and substrates have been measured by the appearance or disappearance of reduced diphosphopyridine nucleotide or reduced triphosphopyridine nucleotide. In fact, with the aid of auxiliary enzymes nearly every substance of biological interest could be measured with a pyridine nucleotide system