THE DETERMINATION OF ASCORBIC ACID IN SMALL AMOUNTS OF BLOOD SERUM
Abstract
The dinitropheriylhydrazine method of Roe add Kuether (Abst. 261, Vol. 13) was adapted for estimating ascorbic acid in 0.01 ml. of serum. The values obtained were in agreement with those obtained by the indophenol macromethod of Mindlin and Butler (Abst. 390, Vol. 8). Necessary changes in procedure for adaptation to microanalysis appeared to represent simplifications applicable to macro-analysis as well. The micromethod is particularly suited for nutritional surveys owing to the ease of obtaining suitable blood specimens, the stability of the specimens, and convenience for making analyses on a large scale. L. W. Mapson.
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