Protein Measurement with the Folin Phenol Reagent

Abstract

1. A study is presented of the measurement of proteins with the Folin phenol reagent after alkaline copper treatment. The basic reactions have certain peculiarities which need to be taken into consideration in using this reagent. 2. Directions are given for measurement of proteins in solution and proteins which have been precipitated with acid, etc. A micro procedure is also described for the measurement of as little as 0.2 γ of protein. 3. The differences in the amount of color obtained with a number of proteins is recorded. Interfering substances are listed.