Changes in the activity of Sucrose Metabolizing Enzymes During the Germination of Wheat Seeds

Abstract

Changes in the activities of acid invertase and sucrose synthase during germination of wheat (Triticum aestivum L. cv. Kanchan) seeds were investigated. The activities of both acid invertase and sucrose synthase were changed during growth of seedlings. Until third day of germination their activities increased and thereafter decreased. The activity of acid invertase in the soluble fraction was higher than that of cell wall- bound fraction and for soluble fraction coleoptiles showed a significantly higher acid invertase activity than roots. But in the cell wall-bound fraction a higher activity was found in roots. Sucrose synthase activity was also higher in roots than in coleoptiles. Sucrose content was negatively correlated with the acid invertase activity in both coleoptiles and roots accounting well for the relation between substrate and the activity. Fructose content was significantly higher than the glucose and sucrose content and the amount of sugar was higher in roots than in coleoptiles.