Paper Title
Purification, characterization and some studies on secondary structure of tannase from Aspergillus awamori nakazawa
Keywords
- Tannase Purification
- Tannin Acyl Hydrolase
- Aspergillus Awamori Nakazawa
- Enzyme Characterization
- Fermentation Optimization
- Substrate Combinations
- Media Combinations
- Acetone Precipitation
- Gel Filtration Chromatography
- Enzyme Activity
- Temperature Optimization
- pH Optimization
- Enzyme Inhibition
- Urea Effect
- Surfactant Effect
- Chelator Effect
- Sodium Lauryl Sulphate Inhibition
- EDTA Inhibition
- Glycoprotein Characterization
- Circular Dichroism Analysis
- Secondary Structure Studies
- β-Sheet Structure
- Globular Enzyme
- Industrial Enzyme Applications
- Enzyme Stability
- Microbial Enzymes
- Bioprocess Optimization
- Biotechnological Applications
Journal
Research Impact Tools
Publication Info
Volume: 40 | Issue: 10 | Pages: 3251-3254
Published On
October, 2005
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Abstract
Tannase (tannin acyl hydrolase EC 3.1.1.20) produced by Aspergillus awamori nakazawa was purified and characterized. Optimal conditions of production were determined using varying substrate combinations and studying fermentation on various media combinations. Fermentation was carried out for 46 h for optimum enzyme production. Enzyme samples were obtained from the broth after fermentation by acetone precipitation of the supernatant followed by gel filtration chromatography. The properties of the enzyme were investigated. The optimum conditions of temperature and pH were investigated and the effects of urea, surfactant and chelator were studied. Tannase from this new isolate exhibited optimum activity at 35 °C and at a pH of 5.0. Urea concentrations higher than 3 M were inhibitory. Increasing concentrations of sodium lauryl sulphate also led to decrease in activity. Two percent SLS was inhibitory. Increasing concentrations of EDTA had an inhibitory effect on tannase. Tannase was found to be a glycoprotein. Circular dichroism analysis of purified fractions of tannase indicates that the β-sheet structure in tannase is predominant indicating its globular nature.
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