The study was carried out to survey of fungi associated with the determination of fruits, vegetables and betel leaves were collected and two strains of pathogenic fungi- Aspergillus niger and A. flavus were isolated, identified from 45 collected samples in two different zones (southwestern and central) of Bangladesh. In betel leaves, both isolated fungi were showed their highest percentage of frequency (A. niger- 66.66% and A. flavus- 46.66%) where A. niger showed more pathogenic than A. flavus. The result of the pathogenicity test indicates that all the isolated fungi were pathogenic to their respective samples, except orange. The two species of Aspergillus spp. were found to be associated as the predominant fungi with the rotten fruits, vegetables and betel leaves.