Paper Title
Microbial dynamics during anaerobic digestion of sewage sludge combined with food waste at high organic loading rates in immersed membrane bioreactors
Keywords
- Anaerobic Digestion
- Microbial Profiling
- Sewage Sludge
- Food Waste
- Volatile Fatty Acids
- Immersed Membrane Bioreactor
- Organic Loading Rate
- Firmicutes
- Actinobacteria
- Proteobacteria
- Clostridium
- Lactobacillus
- Acetate Production
- Butyrate Production
- Caproate Production
- Lactate Production
- Clostridium sp. W14A
- Bacterium OL-1
- Lactobacillus Mucosae
- Bacterium MS4
- Microbial Community
- Environmental Factors
- Redundancy Analysis
- Network Analysis
- Acetogenic Bacteria
- VFAs Generation
- Bioreactor Optimization
- Waste-to-Energy
- Biomass Valorization
- Sustainable Waste Management
Journal
Research Impact Tools
Publication Info
Volume: 303 | Pages: 121276
Published On
November, 2021
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Abstract
This study was designed to evaluate the microbial profiling of anaerobic digestion during the processing of sewage sludge and food waste to volatile fatty acids (VFAs) in an immersed membrane bioreactor (iMBR) operating with a distinct organic loading rate (OLR). The results indicated that Firmicutes (0.17–0.38) and Actinobacteria (0.20–0.32) phyla dominated in anaerobic digestion with OLRs of 4 and 8 g VS/L/d, while Firmicutes (0.04–0.08), Actinobacteria (0.03–0.08) and Proteobacteria (0.02) were more abundant with OLR of 6 and 10 g VS/L/d in the bioreactors. Subsequently, the abundance of the Clostridium and Lactobacillus genera were responsible for higher yields of acetate, butyrate, caproate and lactate. The species of Clostridium sp. W14A (0.04–0.06), Bacterium OL-1(0.01–0.30) and Lactobacillus mucosae (0.002–0.01) were rich for both OLR dosages. Additionally, network and redundancy analysis confirmed that Clostridium sp. W14A, Bacterium MS4 and Lactobacillus had significant correlations with the VFAs produced, such as acetate, butyrate, and caproate. Variation analysis also demonstrated an appreciable correlation between environmental factors and the bacterial community. Overall, this bacterial community was dominated by the Firmicutes (0.04–0.38) phylum and Clostridium sp. W14A (0.04–0.60) species, which is a clear indicator of a lower population of acetogenic bacteria associated with greater VFAs generation.
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