Out of 150 burn wound swabs samples taken from hospitalized patients in city hospital, Bangladesh patients, 100 samples were found positive by bacterial infection who presented invasive burn wound infection from both sex and average age of 10-55 years. Pseudomonas aeruginosa was found to be the most common isolate (23.33%) followed by Staphylococcus aureus (15.33%), Enterobacter spp. (8.66%), Proteus vulgaris (8%), Micrococcus sp. (3.33%), E. coli (4.66%) and Klebsiella spp. (3.33%). Among 8 antibiotics, antibiotic sensitivity pattern of Ciprofloxacin was found to be the most effective drug against most of the Gram-negative and Gram-positive isolates followed by Amikacin, while Chloramphenicol, Doxycycline and Gentamicin were less sensitive to few isolates.