Urinary Tract Infection (UTI) is the problem for the hospitalized patients as Nosocomial infection and threat for the healthy peoples. Most of the Gram negative organisms are responsible for UTI but some Gram positive bacteria also cause UTI which is more in female than male. In this study, twelve organisms were found that causing UTI. The most common bacteria among the isolates which cause UTI was E. coli (53.8%). Moreover, 51-60 age group females (14.5%) and 75.3% OPD patients were found to be more prone to UTI. Most effective antibiotics sensitive against E. coli were Meropenem, Amikacin, Nitrofurantoin and Piparacillin / Tazobactam.