FASEB JOURNAL PAULITHA PASALA Effect of Streptozocin Article

Abstract

Studies have focused on gastric dysmotility in diabetes mellitus. Effects of hyperglycemia on colonic motility and secretion are not known. 5‐HT was reported to mediate contractility by activating receptors on enteric neurons and smooth muscle. This study investigated effects of 5‐HT on contractility coordinated with secretion in diabetic rats. Sonomicrometry and voltage clamp techniques were used to measure motility and secretion. Sprague Dawley rats were injected with streptozocin (65 mg/kg bw) in the tail veins. Rats were sacrificed 10–12 days following the induction of hyperglycemia (blood glucose 300–400 mg/dl). Sheets of colon were mounted in Ussing chambers. 1 mm piezocrystals were glued to serosal surface 4–5 mm apart. Circular contractions as decrease in inter‐crystal distances (ICD) were measured. Voltage‐clamping 0 mV was used for short circuit current (Isc).