Paper Title
Experimental design to enhance the production of l-(+)-lactic acid from steam-exploded wood hydrolysate using Rhizopus oryzae in a mixed-acid fermentation
Keywords
- L-(+)-Lactic Acid Production
- Rhizopus Oryzae
- Mixed-Acid Fermentation
- Steam-Exploded Wood Hydrolysate
- Pinus Taeda Hydrolysate
- Hemicellulosic Hydrolysate
- Reducing Sugar Conversion
- Fermentation Optimization
- Experimental Design
- Factorial Design
- Surface Response Methodology
- Agitation Level
- Substrate Concentration
- CaCO3 Concentration
- Carbon-Nitrogen Ratio
- Carbon-Phosphorus Ratio
- Statistical Analysis
- Biological Conversion
- Fungal Fermentation
- Bioprocess Optimization
- Renewable Feedstock Fermentation
- Sustainable Bioproduction
- Lactic Acid Biotechnology
- Fermentation Parameter Optimization
- Industrial Microbiology
- Bio-Based Chemicals
Journal
Research Impact Tools
Publication Info
Volume: 34 | Issue: 9 | Pages: 949-955
Published On
October, 1999
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Abstract
The fermentation of hemicellulosic hydrolysate from Pinus taeda chips, using the fungal culture Rhizopus oryzae, was carried out to produce l-(+)-lactic acid and to optimize and enhance the biological conversion of reducing sugar into l-(+)-lactic acid using the experimental design to evaluate the culture conditions. The first factorial design based on surface response with five factors (agitation level, substrate concentration, CaCO3 concentration, C/N and C/P ratios) at low levels and one medium point was performed to optimize culture conditions. The second study tested two factors (substrate concentration and C/N ratio) at three levels. The statistical analysis of the data obtained from the factorial study showed that a C/N ratio of 35 and substrate concentration of 90 g/litre were the best conditions to produce l-(+)-lactic acid with R. oryzae on P. taeda hydrolysate, but in this case the statistical projection was not correct and the real optimized conditions were C/N ratio of 55 and substrate concentration of 75 g/litre of reducing sugar.
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