In the present study, the bacterial consortia of three combinations were used and identification of unknown bacteria was carried out. The combination of 3PRRF5b+4DTF1b+10DTW2b was found to have better retting performance. Formulations showed good potential as candidates for the microbial consortium. Selected microbes were screened out by gram staining. Growth and colony characteristics were determined and the Gram-Negative and Positive selection criteria of unknown bacteria were done. The possible dichotomous identification maps and dendrogram for Gram-Negative and Gram-Positive organisms were measured for probable detection of the isolates. On the biochemical analysis, the organisms can match up to 80% till genus level but this research needs to be optimized for the identification of these consortia.