The study was projected to application and fastness analysis of natural dyes on cotton knitted fabric with references to measure color fastness to four criteria; like color fastness to wash, color fastness to perspiration, color fastness to light and color fastness to rubbing. Test was carried out for 30min at 60oC and grading was done for color staining and color change by the respective grey scale. But change in color grading was very poor; the lowest grade was found for turmeric and combination of tea and henna was 1. The rubbing fastness of the sample cloth and degree of staining is accessed both for wet and dry rub by grey scale. Color fastness to light was carried out in the Xenon Arc. Dry rub grading was 5 and Wet rub grading was 4. Rubbing fastness properties of these natural dyes were comparatively poor and the results of the grading for light fastness were very poor.