ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF RIZATRIPTAN BENZOATE TABLETS BY RP-LC

Abstract

This paper describes the analytical method suitable for validation of Rizatriptan benzoate by reversed Phase liquid chromatography (RP-LC) method. The method utilized RP-LC (Water 2695 with UV detector) model and a column L1, 250mm ´ 4.6 mm, 5m (Inertsil, ODS- 3V, 250mm ´ 4.6mm, 5m). The mobile phases were comprised of A, B of Acetonitrile and Buffer pH (6.5).validation experiments were performed to demonstrate System suitability, specificity, precision, linearity and Range, Accuracy study, stability of analytical solution and robustness. The method was linear over the concentration range of 30-70 mg/ML-1. The method showed good recoveries (96.64 - 97.71%) and the relative standard deviations of intra and inter-day assay were 97.4% and 96.20% respectively. The method can be used for quality control assay of Rizatriptan benzoate.