In this experiment, tea liquor has been prepared by 10% dried tea leaves and boiling time was 5, 10 and 15 minutes. For the determination of antibacterial activity, Salmonella typhi were inoculated on nutrient agar media by spread plate method and tea liquor was poured in the middle of the bacterial inoculated plate. Antibacterial activity was observed where boiling time has affected antibacterial activity. Antibacterial activity of different extracts of jute leaves were evaluated by agar well diffusion method against different pathogenic bacteria viz., Escherichia coli, Pseudomonas aeruginosa, Bacillus cereus etc. Antibacterial activity was found increasing with increased by longer time prepared tea liquor and the highest antibacterial activity of the aqueous extract of jute leaf was observed against E. coli (ZOI=17.0±1.0mm) followed by Salmonella typhi. Therefore, these results clearly support the traditional use of jute leaf as a broad-spectrum antimicrobial agent against a wide range of microbes.