Jyoti V Thaikoottathil Research Profile

Publication

Citations

Editorial Contribution

Network

Conferences/Seminar

About

I have research expertise in airway cell biology, lung diseases such as asthma,COPD, acute lung injury, innate immunity, gene regulation and cancer biology. I have strong interest in building translational research program involving effects of environmental toxicant / pollutant exposure on human health with special interest in lung and heart diseases. Currently I am monitoring in vitro regulatory toxicity studies (Non-GLP & GLP) at extrenal CROs. Research Experience - Basic and translational research focused on inflammatory lung diseases such as asthma, COPD, respiratory infections. Antimicrobial protein/ gene regulation in airway epithelium. Preclinical drug development (in vitro ADMET studies) < 2 yrs , Regulatory in vitro toxicity studies <1 yr. Effects of environmental toxicant exposures (cigarette smoke, ambient fine particles and metal oxide nanoparticles) on human health with regards to lung and heart disease (>8 yrs). Adenoid cystic carcinoma (ACC) studies using primary tumour cells and mouse xenograft (PDX) models (4 yrs). Specialties: Airway innate immunity, allergic asthma and infection, Airway cell biology, cell signalling, transcriptional regulation of genes, Acute lung injury, Oncology (solid tumour), Human aortic endothelial cell biology study with fluid shear stress and environmental toxicant exposures. Regulatory in vitro toxicology.

Skills

Experience

Associate Professor

Institute of Advanced Research & University for Innovation (IAR)

Jul-2022 to Present

Cell Culture · Airway epithelial cell biology

Postdoctoral fellow

University of California Davis

Nov-2007 to Oct-2009

Human primary airway cell isolation and 3D culture, human primary aortic endothelial cell culture, study design, execution, data analysis, interpretation, presentation, scientific writing, leading collaborative/ cross-functional research projects. Metal oxide nanoparticle and fine ambient particle induced inflammation in primary human aortic endothelial cells and their role in developing atherosclerosis. Modulation of primary human aortic endothelial cell inflammatory response to metal oxide nanoparticles and ambient particles by steady and oscillatory flow/shear stress. Attenuation of mucus metaplasia by rad, a small GTPase binding protein in airway epithelial cells. Generation of lung specific Rad transgenic mouseHuman primary airway cell isolation and 3D culture, human primary aortic endothelial cell culture, study design, execution, data analysis, interpretation, presentation, scientific writing, leading collaborative/ cross-functional research projects. Metal oxide nanoparticle and fine ambient particle induced inflammation in primary human aortic endothelial cells and their role in developing atherosclerosis. Modulation of primary human aortic endothelial cell inflammatory response to metal oxide nanoparticles and ambient particles by steady and oscillatory flow/shear stress. Attenuation of mucus metaplasia by rad, a small GTPase binding protein in airway epithelial cells. Generation of lung specific Rad transgenic mouse Skills: Cell Culture · Airway epithelial cell biology · Biochemistry

Postdoctoral Research Associate

National Jewish Health, Denver

May-2006 to Nov-2007

Human primary airway cell 3D culture, study design, execution, data analysis, interpretation, presentation, scientific writing. Host-pathogene interaction:Mycoplasma pneumoniae & lung epithelium cells. Modulation of innate immune system by cigarette smoke and allergic milieu with especial focus on lung airway epithelium.Human primary airway cell 3D culture, study design, execution, data analysis, interpretation, presentation, scientific writing. Host-pathogene interaction:Mycoplasma pneumoniae & lung epithelium cells. Modulation of innate immune system by cigarette smoke and allergic milieu with especial focus on lung airway epithelium. Skills: Cell Culture · Airway epithelial cell biology · ELISA · Biochemistry

Postdoctoral Researcher

University of California Davis

Dec-2004 to May-2006

Human lung cell line and macrophage cell line culture, study design, execution, data analysis, interpretation, presentation, scientific writing. Role of ceramide-induced apoptosis and signal transduction mechanism in alveolar macrophages and lung epithelial cells and its modulation by oxidants and environmental toxicants. Human lung cell line and macrophage cell line culture, study design, execution, data analysis, interpretation, presentation, scientific writing. Role of ceramide-induced apoptosis and signal transduction mechanism in alveolar macrophages and lung epithelial cells and its modulation by oxidants and environmental toxicants. Skills: Cell Culture · Airway epithelial cell biology · Biochemistry

Assistant Professor

The Maharaja Sayajirao University of Baroda (MSUB)

Oct-2020 to Jun-2022

Research Associate

University of Virginia (UVA)

Aug-2014 to Jul-2018

Human primary tumor cell isolation from PDX, cell characterization and culture, study design, execution, data analysis, interpretation, presentation, scientific writing, leading collaborative/ cross-functional research projects. Study the role of MYB in adenoid cystic carcinoma (ACC) pathology using xenograft (PDX) and primary ACC cell culture models and explore its potential to design a targeted therapy for ACC. Evaluation of anti-cancer activity of small molecule inhibiotrs and peptides in primary human ACC cellsHuman primary tumor cell isolation from PDX, cell characterization and culture, study design, execution, data analysis, interpretation, presentation, scientific writing, leading collaborative/ cross-functional research projects. Study the role of MYB in adenoid cystic carcinoma (ACC) pathology using xenograft (PDX) and primary ACC cell culture models and explore its potential to design a targeted therapy for ACC. Evaluation of anti-cancer activity of small molecule inhibiotrs and peptides in primary human ACC cells Skills: Cell Culture · Biochemistry

Assistant Professor

The Maharaja Sayajirao University of Baroda (MSUB)

Jul-2013 to Jul-2014

Teaching undergraduate and postgraduate students Cell Biology and Biochemistry.

Postdoctoral fellow

National Jewish Health, Denver

Apr-2012 to Jul-2013

Study the role of ADAM17/NRG-1/HER2 signaling in causing acute lung injury (ALI) using various in vitro and in vivo study models. Investigate the detailed mechanism of HER2, a tyrosine kinase receptor mediated changes in Apical junctional complex proteins and its role in maintaining the alveolar barrier function.Study the role of ADAM17/NRG-1/HER2 signaling in causing acute lung injury (ALI) using various in vitro and in vivo study models. Investigate the detailed mechanism of HER2, a tyrosine kinase receptor mediated changes in Apical junctional complex proteins and its role in maintaining the alveolar barrier function. Skills: Cell Culture · Airway epithelial cell biology · ELISA · Biochemistry

Senior Scientist II

Altria Client Services, Richmond

Jul-2018 to Feb-2019

In vitro regulatory toxicity studies (Non-GLP & GLP) at external CROs New assay development (Mechanistic assays), Scientific writing and review - study reports, manuscript/ abstracts/ poster.

Postdoctoral Fellow & Instructor

National Jewish Health, Denver

Oct-2009 to Apr-2012

Human primary airway cell isolation and 3D culture, study design, execution, data analysis, interpretation, presentation, scientific writing, leading collaborative/ cross-functional research projects. Regulation of SPLUNC1 gene expression under basal and diseased conditions. Determine novel immuno-modulatory function of antimicrobial protein SPLUNC1 under basal and allergic inflammation conditions.Human primary airway cell isolation and 3D culture, study design, execution, data analysis, interpretation, presentation, scientific writing, leading collaborative/ cross-functional research projects. Regulation of SPLUNC1 gene expression under basal and diseased conditions. Determine novel immuno-modulatory function of antimicrobial protein SPLUNC1 under basal and allergic inflammation conditions. Skills: Cell Culture · Airway epithelial cell biology · ELISA · Biochemistry

Research Scientist

BioArc Research Solutions

Apr-2003 to Apr-2004

nvitro ADME/T studies for CRO. Experiment design and execution for different CYP450 isoforms inhibition study and IC 50 determination for different chemicals/ potential drugs. Experiments design and execution to determine drug metabolism and half life by human liver microsomes, human liver S9 fraction, rat liver microsomes, recombinant CYP3A4 and CYP2D6 etc. Helped in lab designing for Bio Arc Research Solutions. Assay documentation, SOP, validation report and other report preparation etc