Jyoti V Thaikoottathil

Associate Professor

• Cell Culture · Airway epithelial cell biology

Assistant Professor

Senior Scientist II

• In vitro regulatory toxicity studies (Non-GLP & GLP) at external CROs New assay development (Mechanistic assays), Scientific writing and review - study reports, manuscript/ abstracts/ poster.

Research Associate

• Human primary tumor cell isolation from PDX, cell characterization and culture, study design, execution, data analysis, interpretation, presentation, scientific writing, leading collaborative/ cross-functional research projects. Study the role of MYB in adenoid cystic carcinoma (ACC) pathology using xenograft (PDX) and primary ACC cell culture models and explore its potential to design a targeted therapy for ACC. Evaluation of anti-cancer activity of small molecule inhibiotrs and peptides in primary human ACC cellsHuman primary tumor cell isolation from PDX, cell characterization and culture, study design, execution, data analysis, interpretation, presentation, scientific writing, leading collaborative/ cross-functional research projects. Study the role of MYB in adenoid cystic carcinoma (ACC) pathology using xenograft (PDX) and primary ACC cell culture models and explore its potential to design a targeted therapy for ACC. Evaluation of anti-cancer activity of small molecule inhibiotrs and peptides in primary human ACC cells Skills: Cell Culture · Biochemistry

Assistant Professor

• Teaching undergraduate and postgraduate students Cell Biology and Biochemistry.

Postdoctoral fellow

• Study the role of ADAM17/NRG-1/HER2 signaling in causing acute lung injury (ALI) using various in vitro and in vivo study models. Investigate the detailed mechanism of HER2, a tyrosine kinase receptor mediated changes in Apical junctional complex proteins and its role in maintaining the alveolar barrier function.Study the role of ADAM17/NRG-1/HER2 signaling in causing acute lung injury (ALI) using various in vitro and in vivo study models. Investigate the detailed mechanism of HER2, a tyrosine kinase receptor mediated changes in Apical junctional complex proteins and its role in maintaining the alveolar barrier function. Skills: Cell Culture · Airway epithelial cell biology · ELISA · Biochemistry

Postdoctoral Fellow & Instructor

• Human primary airway cell isolation and 3D culture, study design, execution, data analysis, interpretation, presentation, scientific writing, leading collaborative/ cross-functional research projects. Regulation of SPLUNC1 gene expression under basal and diseased conditions. Determine novel immuno-modulatory function of antimicrobial protein SPLUNC1 under basal and allergic inflammation conditions.Human primary airway cell isolation and 3D culture, study design, execution, data analysis, interpretation, presentation, scientific writing, leading collaborative/ cross-functional research projects. Regulation of SPLUNC1 gene expression under basal and diseased conditions. Determine novel immuno-modulatory function of antimicrobial protein SPLUNC1 under basal and allergic inflammation conditions. Skills: Cell Culture · Airway epithelial cell biology · ELISA · Biochemistry

Postdoctoral fellow

• Human primary airway cell isolation and 3D culture, human primary aortic endothelial cell culture, study design, execution, data analysis, interpretation, presentation, scientific writing, leading collaborative/ cross-functional research projects. Metal oxide nanoparticle and fine ambient particle induced inflammation in primary human aortic endothelial cells and their role in developing atherosclerosis. Modulation of primary human aortic endothelial cell inflammatory response to metal oxide nanoparticles and ambient particles by steady and oscillatory flow/shear stress. Attenuation of mucus metaplasia by rad, a small GTPase binding protein in airway epithelial cells. Generation of lung specific Rad transgenic mouseHuman primary airway cell isolation and 3D culture, human primary aortic endothelial cell culture, study design, execution, data analysis, interpretation, presentation, scientific writing, leading collaborative/ cross-functional research projects. Metal oxide nanoparticle and fine ambient particle induced inflammation in primary human aortic endothelial cells and their role in developing atherosclerosis. Modulation of primary human aortic endothelial cell inflammatory response to metal oxide nanoparticles and ambient particles by steady and oscillatory flow/shear stress. Attenuation of mucus metaplasia by rad, a small GTPase binding protein in airway epithelial cells. Generation of lung specific Rad transgenic mouse Skills: Cell Culture · Airway epithelial cell biology · Biochemistry

Postdoctoral Research Associate

• Human primary airway cell 3D culture, study design, execution, data analysis, interpretation, presentation, scientific writing. Host-pathogene interaction:Mycoplasma pneumoniae & lung epithelium cells. Modulation of innate immune system by cigarette smoke and allergic milieu with especial focus on lung airway epithelium.Human primary airway cell 3D culture, study design, execution, data analysis, interpretation, presentation, scientific writing. Host-pathogene interaction:Mycoplasma pneumoniae & lung epithelium cells. Modulation of innate immune system by cigarette smoke and allergic milieu with especial focus on lung airway epithelium. Skills: Cell Culture · Airway epithelial cell biology · ELISA · Biochemistry

Postdoctoral Researcher

• Human lung cell line and macrophage cell line culture, study design, execution, data analysis, interpretation, presentation, scientific writing. Role of ceramide-induced apoptosis and signal transduction mechanism in alveolar macrophages and lung epithelial cells and its modulation by oxidants and environmental toxicants. Human lung cell line and macrophage cell line culture, study design, execution, data analysis, interpretation, presentation, scientific writing. Role of ceramide-induced apoptosis and signal transduction mechanism in alveolar macrophages and lung epithelial cells and its modulation by oxidants and environmental toxicants. Skills: Cell Culture · Airway epithelial cell biology · Biochemistry

Research Scientist

• nvitro ADME/T studies for CRO. Experiment design and execution for different CYP450 isoforms inhibition study and IC 50 determination for different chemicals/ potential drugs. Experiments design and execution to determine drug metabolism and half life by human liver microsomes, human liver S9 fraction, rat liver microsomes, recombinant CYP3A4 and CYP2D6 etc. Helped in lab designing for Bio Arc Research Solutions. Assay documentation, SOP, validation report and other report preparation etc