About
Performance and results driven research professional with more than 13 years of experience in microbiology, cellular biology and molecular biology. Proficient at development of recombinant tetravalent dengue DNA vaccine and its immunogenicity and efficacy in mice. Extensive experience in handling various antibody engineering projects. Strengths include excellent time management, collaboration, self -motivation and organization skills. An effective communicator with excellent relationship building and interpersonal skills. A quick learner with the ability to work under pressure and meet deadlines. Seeking a suitable position to utilize and enhance the existing leadership /interpersonal skills and to work in an environment that offers professional growth while being resourceful, innovative and flexible
Skills & Expertise
Microbiology
Cellular Biology
Molecular Biology
Recombinant DNA
Vaccine Development
Immunogenicity
Efficacy
Antibody Engineering
PCR
ELISA
Cell Culture
Virus Propagation
RNA Isolation
cDNA Preparation
Agarose Gel Electrophoresis
Restriction Digestion
Cloning
Transformation
Transfection
Bacteriophage Pannning
Recombinant Protein Expression
Purification
SDS-PAGE
Western Blot
In Vitro Microbiological Assays
Clinical Trial Operations
GCP
GCLP
Data Management
Informatics
Medical Microbiology
Molecular Biology
Detailed Documentation
Training
Team Management
qRT-PCR
Cancer Biomarkers
Antivirals
Influenza A-H1N1
Human Beta Corona Virus Testing
Antiviral and Anticancer Projects
Trueprep Real Time PCR Device
PBMC Isolation
Molecular Assays
Manual Purification by Ni NTA Column
Mice Immunization
LD50 Determination
TCID50 Determination
Neutralizing Antibody Titre Determination
Cytokine Genes Expression Determination
Mice Challenge Studies
Cell Culture-Based Assays
QC Testing
Vaccine Products
Water Samples
BET
OSHA Standards
Database
Gram Staining
Rapid HIV ELISA
RPR
DGI
VDRL
Bacterial Culture
HIV Prevention Trial Unit of HPTN
HIV Vaccine Trial Network (HVTN)
ELISA
Antimicrobial Assay
Basic Microbiological Techniques
Real Time PCR
Plasmid Isolation
RE Digestion
Competent Cells Preparation
MTT Assay
HA and HAI Assay
ELISPOT
Flow Cytometry
Indirect Immunofluorescence Assay
Intracerebral
Intramuscular Inoculation
Bacterial Endotoxin Test
IQC
EQC
SOP Preparation
Research Interests
Time Management
Collaboration
Self-Motivation
Organization Skills
Communication
Relationship Building
Interpersonal Skills
Quick Learner
Work Under Pressure
Meet Deadlines
Leadership
Connect With Me
Experience
Section Technical Co-Ordinator
Monitoring of qRT-PCR Lab, qRT-PCR testing, report release of COVD19 samples
Senior Scientist -Molecular Biology
Anticancer testing -cancer biomarkers and antivirals testing etc., Real -Time PCR and ELISA for cancer and other biomarkers and Influenza A -H1N1, human Beta Corona virus testing etc., Successfully completed various antiviral and anticancer projects.
Application Specialist -Support
Presale Demo, Installation, Training and Troubleshooting of Trueprep Real Time PCR device for Rest of Maharashtra region (W)
Research Associate
Systematically supervising various projects on recombinant antibody engineering for cancer, infectious diseases, autoimmune diseases etc., Responsible for carrying out PBMC isolation, molecular assays - RNA isolation, cDNA preparation, PCR, Agarose gel electrophoresis, Restriction digestion, Cloning , transformation, transfection, bacteriophage panning, ELISA, Recombinant protein expression and manual purification by Ni NTA column , SDS -PAGE, Western Blot in vitro microbiological assays and cell culture -based assays, Detailed documentation of data, research process & procedures for future references, Diligently assisting in preparation of weekly, monthly progress reports and p resentation to the Director, Conscientiously served as a lead to junior team members and as a li nk to other teams by providing assistance and training
Senior Research Fellow
Development of recombinant tetravalent dengue vaccine and study its immunogenicity and efficacy in mice, Work involved cell culture, virus propagation, RNA isolation, RT PCR, Agarose gel electrophoresis, Restriction digestion, Cloning , transformation, transfection, SDS -PAGE, Western Blot, mice immunization, LD 50 determination, TCID 50 determination, neutralizing antibody titre determination, cytokine genes expression determination by Real Time PCR, mi ce challenge studies (efficacy) and cell culture -based assays ., Authored and presented exploratory research proposal to the internal pr oject management and leadership teams
Education
Haffkine Institute for Training, Research & Testing, Mumbai / Maharashtra University of Health Sciences , Nashik
Ruby Hall Clinic and PPCE , Pune
Government Medical College Miraj/ Shivaji University , Kolhapur
S.G.M. College, Karad/ Shivaji University , Kolhapur
Conferences & Seminars (1)
National Virology Conference
Publications (4)
dd