Paper Title

Statistical optimization of simultaneous saccharification and l(+)-lactic acid fermentation from cassava bagasse using mixed culture of lactobacilli by response surface methodology

Keywords

  • Lactic Acid Production
  • L(+)-Lactic Acid
  • Cassava Bagasse
  • Simultaneous Saccharification And Fermentation
  • Lactobacillus Casei
  • Lactobacillus Delbrueckii
  • Box-Behnken Design
  • Process Optimization
  • Enzyme Concentration
  • Yeast Extract
  • NH4Cl
  • Inoculum Size
  • Fermentation Parameters
  • Microbial Fermentation
  • Bioprocess Engineering
  • Bio-Based Chemicals
  • Renewable Feedstock
  • Fermentation Technology
  • Static Culture
  • High-Yield Fermentation
  • Bioconversion
  • Sustainable Bioprocessing
  • Enzyme Mixture
  • CFU/L
  • Fermentation Time
  • Temperature Optimization
  • Industrial Biotechnology

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Publication Info

Volume: 36 | Issue: 6 | Pages: 262-267

Published On

October, 2007

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Abstract

Optimization of five process parameters (concentrations of cassava bagasse, enzyme, yeast extract, NH4Cl and inoculum) was attempted using a Box-Behnken design for the optimal production of l(+)-lactic acid by a mixed culture of Lactobacillus casei and Lactobacillus delbrueckii by simultaneous saccharification and fermentation. Maximum lactic acid yield of 81 g/L was obtained when 15% (w/v) cassava bagasse treated with 12.5 mL/L enzyme mixture was supplemented with 7.5 g/L yeast extract and 3 g/L NH4Cl and inoculated with 3 × 1010 CFU/L of lactobacilli and incubated for 60 h at 37 °C as static culture.

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