Paper Title

Scale-up of biotransformation process in stirred tank reactor using dual impeller bioreactor

Authors

Profile picture of Aniruddha Bhalchandra Pandit
Aniruddha Bhalchandra Pandit

Keywords

  • Scale-Up
  • Biotransformation Process
  • Stirred Tank Reactor
  • Dual Impeller Bioreactor
  • Gas-Liquid Mass Transfer
  • Mass Transfer Coefficient
  • KLa
  • Energy Dissipation
  • Physico-Chemical Properties
  • Disc Turbine
  • Pitched Blade Turbine
  • Gas Hold-Up
  • Yeast Isolate VS1
  • Benzaldehyde Biotransformation
  • l-Phenyl Acetyl Carbinol
  • l-PAC Production
  • Shake Flask Studies
  • Impeller Speed Optimization
  • Aeration Optimization
  • Bioreactor Performance
  • Fermentation Process
  • Industrial Bioprocessing
  • Biochemical Engineering
  • Process Optimization
  • Bioreactor Design
  • Bioprocess Scale-Up
  • Oxygen Transfer
  • Microbial Fermentation
  • Bioprocess Intensification
  • Advanced Fermentation Technology

Journal

Biochemical Engineering Journal External link

Research Impact Tools

DOI

DOI Icon 10.1016/S1369-703X(00)00130-3

Publication Info

Volume: 8 | Issue: 1 | Pages: 19-29

Published On

July, 2001

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Abstract

The gas–liquid mass transfer coefficient KLa in the fermenter is a strong function of mode of energy dissipation and physico-chemical properties of the liquid media. A combination of disc turbine (DT) and pitched blade turbine down flow (PTD) impellers has been tested in laboratory bioreactor for gas hold-up and gas–liquid mass transfer performance for the growth and biotransformation medium for an yeast isolate VS1 capable of biotransforming benzaldehyde to l-phenyl acetyl carbinol (l-PAC) and compared with those in water. Correlations have been developed for the prediction of the fractional gas hold-up and gas–liquid mass transfer coefficient for the above media. The mass transfer coefficient and respiration rate have been determined in the shake flask for the growth as well as for biotransformation medium. These results, then have been used to optimize the operating parameters (impeller speed and aeration) for growth and biotransformation in a laboratory bioreactor. The comparison of cell mass production and l-PAC production in the bioreactor has been done with that obtained in shake flask studies.

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