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Paper Title

RETRACTED: Cellulase production through solid-state tray fermentation, and its use for bioethanol from sorghum stover

Article Type

Research Article

Research Impact Tools

Issue

Volume : 242 | Page No : 265-271

Published On

October, 2017

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Abstract

The production of cellulase by Trichoderma reesei RUT C-30 under solid-state fermentation (SSF) on wheat bran and cellulose was optimized employing a two stage statistical design of experiments. Optimization of process parameters resulted in a 3.2-fold increase in CMCase production to 959.53 IU/gDS. The process was evaluated at pilot scale in tray fermenters and yielded 457 IU/gDS using the lab conditions and indicating possibility for further improvement. The cellulase could effectively hydrolyze alkali pretreated sorghum stover and addition of Aspergillus niger b-glucosidase improved the hydrolytic efficiency 174%, indicating the potential to use this blend for effective saccharification of sorghum stover biomass. The enzymatic hydrolysate of sorghum stover was fermented to ethanol with 80% efficiency.

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