Paper Title
Process intensification of delignification and enzymatic hydrolysis of delignified cellulosic biomass using various process intensification techniques including cavitation
Keywords
- Process Intensification
- Delignification
- Enzymatic Hydrolysis
- Cellulosic Biomass
- Cavitation
- Alkali Pretreatment
- Ultrasound Treatment
- Biological Treatment
- Laccase Enzyme
- Combined Ultrasound-Laccase Treatment
- Napier Grass
- Sodium Hydroxide Pretreatment
- Optimized Conditions
- Lignin Degradation
- Ultrasound Frequency
- Enzyme Concentration
- Impeller Speed
- Treatment Time Reduction
- Biomass Conversion
- Renewable Energy
- Biofuel Production
- Green Processing Techniques
Journal
Research Impact Tools
Publication Info
Volume: 213 | Pages: 162-168
Published On
August, 2016
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Abstract
Different methods of pretreatment including alkali treatment, treatment with ultrasound, biological treatment using laccase enzyme and combined treatment like ultrasound-laccase for Napier grass have been tried. With alkali pretreatment optimized conditions obtained were sodium hydroxide 0.3% w/v giving 86% delignification at temperature of 80 °C, treatment time of 2 h. In physical methods of treatment ultrasound, at a temperature of 45 °C, treatment time of 2 h, operating at frequency 24 kHz and power of 100 W gave 18% delignification. For laccase pretreatment, optimized conditions obtained were 300 rpm impeller speed, enzyme concentration 10 U/gm of Napier grass gave 50% delignification with cellulose. The optimized conditions for delignification by using combination treatment of ultrasound & enzymatic were obtained at 24 kHz frequency, 100 W giving 75% of delignification in 6 h. An enhancement in lignin degradation by 25% and reduction in the treatment time from 12 to 6 h is achieved as compared to only laccase treatment.
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