Paper Title
Pentose-rich hydrolysate from acid pretreated rice straw as a carbon source for the production of poly-3-hydroxybutyrate
Keywords
- Poly-3-Hydroxybutyrate
- PHB Production
- Bacterial Strains
- Bacillus Firmus
- Pentose Sugar Hydrolysate
- Rice Straw
- Acid Pretreatment
- Black Liquor
- Sugar Degradation Products
- Formic Acid
- Acetic Acid
- Furfural
- Hydroxymethyl Furfural
- Biomass Accumulation
- Fermentation Optimization
- Inoculum Concentration
- Xylose Utilization
- Biopolymer Characterization
- FTIR
- 1H NMR
- 13C NMR
- Sustainable Bioplastics
- Biodegradable Polymers
- Microbial Fermentation
- Renewable Carbon Sources
- Industrial Biotechnology
- Green Chemistry
- Fermentation Media
- Detoxification-Free Growth
- High PHB Yield
- Sigma Standard PHB
Journal
Research Impact Tools
Publication Info
Volume: 78 | Issue: 67-72
Published On
September, 2013
Downloads
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Abstract
The aim of this work was to evaluate three different bacterial strains for their ability to accumulate poly-3-hydroxybutyrate (PHB) using pentose sugar rich hydrolysate generated from acid pretreated rice straw as the sole carbon source. Out of these, Bacillus firmus NII 0830 showed maximum PHB production. Acid pretreated black liquor contained sugars and sugar degradation products such as formic acid, acetic acid, furfural and hydroxymethyl furfural. The bacterium grew in the hydrolysate medium without any detoxification and it could accumulate 1.9 g/l biomass with 1.697 g/l PHB and the PHB content in the cell was 89%. This was the highest value ever reported from Bacillus species. The optimum conditions for the fermentation media were an inoculum concentration of 6.5%, 90 h of incubation and 0.75% of xylose concentration. The characterization of extracted polymer was carried out by FTIR, 1H and 13C NMR which showed characteristics similar to that of the standard PHB from Sigma.
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