Paper Title
Isolation of α-glucosidase from Saccharomyces cerevisiae: cell disruption and adsorption
Keywords
- α-Glucosidase Isolation
- Saccharomyces Cerevisiae
- Cell Disruption
- Ultrasonication
- Specific Activity Optimization
- Leached Solution
- pH Optimization
- Unit Activity
- Specific Activity
- pH Influence
- Adsorptive Isolation
- Non-Ionic Polystyrene-Divinylbenzene (XAD-16)
- Surface Modification
- Graft Co-Polymerisation
- Acrylate Monomers
- Adsorptive Purification
- Temperature Effect
- Selective Adsorption
- Storage Stability
- Recycling Ability
- Immobilized Enzyme
Journal
Research Impact Tools
Publication Info
Volume: 15 | Issue: 1 | Pages: 37-45
Published On
July, 2003
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Abstract
Cell disruption of S. cerevisiae was carried out with ultrasonication, under specific operating conditions, to get optimum specific activity for α-glucosidase in a leached solution. The pH of the disruption medium was optimised for both, unit activity and specific activity of α-glucosidase. A high unit activity was obtained at pH 8 (172 units/ml) while the specific activity was at its maximum at pH 6 (232 units/mg). The strongly hydrophobic character of non-ionic polystyrene–divinylbenzene (XAD-16) renders it unsuitable for the adsorptive isolation of certain enzymes/proteins. Surface modification of this support by graft co-polymerisation with a few selected acrylate monomers was recommended in our previous work [1] for the adsorptive purification of α-glucosidase. In the present work, the effect of the parameters, viz., temperature and pH of the medium so as to get both, maximum and selective adsorption of α-glucosidase has been reported. Thus, the present work deals with the results of this optimisation exercise, along with the storage stability and recycling ability of the immobilised enzyme.
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