Paper Title
Improved cavitational cell disruption following pH pretreatment for the extraction of β-galactosidase from Kluveromyces lactis
Keywords
- Cavitational Cell Disruption
- pH Pretreatment
- β-Galactosidase Extraction
- Kluveromyces lactis
- Ultrasonic Disruption
- Enzyme Yield Optimization
- Cell Wall Permeabilization
- Hydrodynamic Cavitation
- Sonication
- Enzyme Recovery
- Bioprocess Optimization
- Fermentation Byproducts
- Intracellular Enzyme Release
- Downstream Processing
- Protein Extraction
- Biochemical Engineering
- Industrial Enzyme Production
- Lactose Hydrolysis
- Dairy Industry Applications
- Yeast Biotechnology
- Energy Efficiency
- Ultrasound-Assisted Extraction
- Pretreatment Effect
- Bioprocess Intensification
- Microbial Enzyme Production
- Process Engineering
Journal
Research Impact Tools
Publication Info
Volume: 31 | Issue: 1 | Pages: 25-30
Published On
August, 2006
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Abstract
The efficient release of intracellular β-galactosidase by ultrasonic disruption of cells treated with aqueous solutions of different pH has been investigated to improve the cavitational efficacy of the cell disruption process. Pretreatment of the cells at different pHs for various time intervals was studied. The maximum yield (95 ± 3 U/ml) was obtained by pretreatment of the cells at pH 4.4 for 6 h and subsequent ultrasonic cell disruption for 40 min. The maximum yield on ultrasonic disruption without pretreatment was 7.2 ± 0.6 U/ml. The energy efficiency of the disruption process for releasing β-galactosidase using different pre-treatments has been calculated and compared.
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