Go Back Research Article December, 2009

Effect of various additives on enzymatic hydrolysis of castor oil

Author Aniruddha Bhalchandra Pandit Author Virendra K. Rathod

Abstract

Hydrolysis of castor oil using lipase enzyme is carried out in a batch reactor at room temperature (35–40 °C). In order to reduce the cost of enzyme catalyzed reaction, water in oil emulsion and a 3:1 ratio of oil to water is selected. The concentration of enzyme in the reaction mixture is optimized. The effect of various additives like solvent and salt which can enhance the rate of reaction is studied. It is found that the glycerol has no effect on the hydrolysis of oil. The reusability of the lipase enzyme has also been tested. The yield of enzymatic hydrolysis of castor oil is compared with those of coconut oil and olive oil.

Keywords

Enzymatic Hydrolysis Castor Oil Lipase Enzyme Batch Reactor Oil Hydrolysis Water-in-Oil Emulsion Oil-to-Water Ratio Enzyme Optimization Additive Effect Solvent Influence Salt Influence Glycerol Effect Reaction Rate Enhancement Enzyme Reusability Yield Comparison Coconut Oil Hydrolysis Olive Oil Hydrolysis Biocatalysis Green Chemistry Sustainable Processing Industrial Enzyme Application Lipid Bioconversion Renewable Resources Fatty Acid Production Biochemical Engineering Emulsion Stability Enzyme Efficiency Renewable Bioproducts Bioprocess Optimization
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Volume 47
Issue 1-3
Pages 93-99
DOI 10.1016/j.bej.2009.07.008
ISSN 1873-295X
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