Determination of Hot Spots in Protein Sequence using Digital Filter

Abstract

The method is used to identify the hot spots from the protein sequence which gives the characteristic frequency. Protein sequence belonging to the functional group of interest is converted into equivalent numerical sequence by assigning EIIP value to each amino acid in the protein sequence. DFT’s of the numerical sequence is computed to obtain their consensus spectrum which leads to the characteristic frequency. This characteristic frequency is selected by filtering the numerical sequence using a specialized narrowband band-pass digital filter. Energy peak is computed from the filter output to select the dominant characteristic frequency from the numerical sequence. These energy peaks from the filtered proteins numerical sequence gives the locations of hot spots. MATLAB is used for implementation of this technique.