Detection of single nucleotide polymorphism by T-ARMS PCR of crossbred cattle Karan Fries for A1, A2 β-casein types

Abstract

Detection of Single nucleotide Polymorphism by T-ARMS PCR of cross bred cattle Karan Fries for A1, A2 beta casein types” to distinguish between A1 and A2 type containing beta-casein and genotyping of hundred Karan Fries cross breed cattle. Casein is the main milk protein where it accounts for 80% of bovine milk protein and contains four fractions (alpha S1-CN, alpha S2-CN, beta-CN and k-CN). Beta casein contributes 25-35% of milk protein and many variants are reported (A1, A2, A3, B, C, D, E, F, G, H1, H2 and I) in different breeds of cattle. The beta casein variants A1 and A2 differs in the 67th amino acid position, the substitution of proline in A2 type with Histidine (in A1) is mainly due to a replacement of “C”