Alpha amylase production by Aspergillus oryzae employing solid-state fermentation

Abstract

This study presents production of a-amylase by Aspergillus oryzae in solid-state fermentation using 14 agro-industrial wastes as substrate. Enzyme production was growth associated and maximum titers (15095 U/gds) were obtained after 72 h when incubated at 30°C on wheat bran (initial moisture content, 60%; initial medium pH, 5). Enzyme titers increased significantly when the solid medium was supplemented with additional N (sodium nitrate) and C (starch) sources.