A Review Article On Ultra Performance Liquid Chromatography (UPLC)

Abstract

UPLC is among the newest developments in liquid chromatography. Ultra Performance Liquid Chromatography is referred to as UPLC. It significantly alters the analysis's sensitivity and speed. Compared to HPLC, its equipment can function at a higher pressure. The ideas and principles of chromatography are covered in this review, along with a comparison of UPLC and HPLC and a list of advanced features. Examples of some of the more current uses of UPLC are also provided here. The chemical and pharmaceutical industries want to increase drug research's effectiveness while achieving better detection, resolution, sensitivity, and selectivity. UPLC, This goal can be accomplished with a modified HPLC method that uses high pressure and tiny particles (less than 2 μm) in the column. Because UPLC, grounded in the Van Deemter equation that correlates linear velocity with plate height, offers benefits such as decreased time and solvent use. This review focuses on the theory, tools, and uses of UPLC, which is an enhanced class of HPLC with better chromatographic performance.