MAHANTHESHKUMAR G T

About

To pursue a highly rewarding career and seeking healthy work environment where I can utilize my skills and knowledge efficiently for organizational growth.

Skills

• Animal Biotechnology
• Cell quantitation by Neubauer counting chamber
• Cell viability by dye exclusion method
• Cell line maintenance
• Preparation of suspension culture of lymphocytes
• Preparation of Balanced Salt Solution
• Preparation of cell culture medium
• Genetics and Molecular Biology
• DNA isolation from bacterial cells
• agarose gel electrophoresis
• SDS-PAGE
• protein and nucleic acid quantification
• restriction digestion
• qPCR
• cDNA preparation
• Southern and Western blotting techniques
• ELISA
• Microbiology
• Negative staining for the bacterial culture
• Simple staining from bacterial culture
• Streak/spread bacterial plate technique
• Recombinant DNA Technology
• Isolation of DNA/RNA/Plasmid from bacterial sources
• Quantification of DNA/RNA
• Agarose/Polyacrylamide Gel electrophoresis
• Restriction digestion DNA
• Ligation of DNA
• Polymerase chain reaction (PCR)
• Immunology
• Immunodiffusion
• Blood Grouping
• Radioimmunoassay
• Lymphocyte Isolation
• RBC And WBC Counting
• Study of Outchterlony double immunodiffusion technique
• Counter current electrophoresis
• Rocket immune electrophoresis
• Biochemistry
• Estimation of Carbohydrates
• Amino acid
• Proteins
• Cholesterol
• Protein purification
• Cell disruption techniques
• Plant tissue culture
• MS media preparation
• Preparation of synthetic seeds
• Isolation of Protoplast
• Anther culture
• Embryo culture
• explant culture and sub culture method
• Meristem culture
• Micropropagation of banana and Vanilla