MAHANTHESHKUMAR G T

Animal Biotechnology Cell quantitation by Neubauer counting chamber Cell viability by dye exclusion method Cell line maintenance Preparation of suspension culture of lymphocytes Preparation of Balanced Salt Solution Preparation of cell culture medium Genetics and Molecular Biology DNA isolation from bacterial cells agarose gel electrophoresis SDS-PAGE protein and nucleic acid quantification restriction digestion qPCR cDNA preparation Southern and Western blotting techniques ELISA Microbiology Negative staining for the bacterial culture Simple staining from bacterial culture Streak/spread bacterial plate technique Recombinant DNA Technology Isolation of DNA/RNA/Plasmid from bacterial sources Quantification of DNA/RNA Agarose/Polyacrylamide Gel electrophoresis Restriction digestion DNA Ligation of DNA Polymerase chain reaction (PCR) Immunology Immunodiffusion Blood Grouping Radioimmunoassay Lymphocyte Isolation RBC And WBC Counting Study of Outchterlony double immunodiffusion technique Counter current electrophoresis Rocket immune electrophoresis Biochemistry Estimation of Carbohydrates Amino acid Proteins Cholesterol Protein purification Cell disruption techniques Plant tissue culture MS media preparation Preparation of synthetic seeds Isolation of Protoplast Anther culture Embryo culture explant culture and sub culture method Meristem culture Micropropagation of banana and Vanilla

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