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Harkirat Singh

Spark 2021 at Bio-NEST
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S9-082024-0902483

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👤 About

Skills & Expertise

Cell Viability Assays Enzyme Assays Immunohistochemistry Cell Culture ELISA Protein Purification PCR DNA Extraction Gel Electrophoresis Aseptic Technique Flow Cytometry Data Analysis Programming (Python, R) Database Management Bioinformatics Data Visualization Genomic Data Analysis

Research Interests

Analytical Curious Organized Adaptable Detail-Oriented Bioinformatics Lab Skills Precision Critical Thinking Creativity

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💼 Experience

Spark 2021

Bio-NEST · 2021 - 2021
Successfully took part in Spark 2021, a major event put out by the Regional Centre of Biotechnology in Faridabad and BBB. Reached the events Grand Finale and displayed great skill and knowledge in the field and demonstrated a strong desire for biotechnology and the capacity to do well in challenging circumstances.

Production and Characterization of Recombinant Oxacillinase protein of in E. Coli

Bennett University · AUG 2023 - DEC 2023
Pioneered research on Acinetobacter baumannii, a multidrug-resistant pathogen, unraveling antibiotic resistance mechanisms. Utilized E. coli expression system for cost-effective production and characterization of oxacillinase proteins. Successfully cloned, expressed, and purified recombinant oxacillinase, providing insights for targeted interventions against multidrug-resistant strains.

NPD Department

Patanjali Padartha · JUN 2023 - AUG 2023
Demonstrated proficiency in molecular and cellular techniques, ensuring accurate data collection, analysis, and interpretation to drive product optimization. Played a key role in maintaining laboratory equipment, ensuring a safe and efficient work environment. I received firsthand knowledge of new product development approaches and testing procedures.

Integrating In silico and wet lab approaches for understanding the structure and function of protein

Bennett University · JAN 2023 - MAY 2023
Conducted a computational evaluation of 100 natural phytochemicals for their potential inhibition of KLF in pancreatic cancer, selecting the top 26 compounds based on their binding affinity. Performed protein purification and analyzed results on SDS-PAGE to assess the efficacy of the selected phytochemicals as potential therapeutic agents in pancreatic cancer treatment.

Nanotechnology

Simplilearn · OCT 2021 - NOV 2021
Demonstrated the significance of Nanotechnology as a field dedicated to material and device construction, particularly highlighting its role in enhancing catalysis to improve the efficiency of fuel synthesis from raw petroleum resources. Successfully completed both a Minor and a Major project, showcasing a strong track record of project management and research skills.

🎓 Education

Bennett University

B.Tech in Biotechnology · 2024

BRD Vidyapeeth

SENIOR SECONDARY SCHOOL in · 2020

BRD Vidyapeeth

SECONDARY SCHOOL in · 2019
Description (between 50 and 1500 characters)

🚀 Projects

Production of beer and wine using bioreacter.
Amplification of DNA using PCR.
Extract DNA from Leaves using SDS method and Visualization by agarose gel electrophoresis.
Performed protein purification and analyzed results on SDS-PAGE.
Docking of Phytochemical using Autodoc and PyRx.
Cell Counting and Viability.
Culture and Maintenance of mammalian Cell lines.

🎤 Conferences & Seminars (2)

International Conference on Pharma and Medical Device Sector
Hot By · City , State , Country · April 2022
International Symposium ADVANCES IN NANOSENSORS & NANOMEDICINE
Hot By · City , State , Country · December 2021
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